Twenty-four heifers were allotted into three groups of eight heifers each and designated control, long-term (LT) or short-term (ST). Animals in the latter two groups were implanted in the ear with 300 mg trenbolone acetate (TBA) for 99 or 62 d, respectively. Four heifers in each group were subjected to liver and fat biopsy 60 d after initiation of the experiment. At slaughter, which occurred 15 d after retrieval of the implant, liver and fat were also collected. The implant was analyzed for residual trenbolone (TBOH), and tissues were analyzed for TBOH and estradiol-17 beta (E2 beta). Blood samples were taken weekly after implantation and daily for 14 d before and after implantation. All heifers were weighed weekly. The ST heifers gained faster (P less than .05) than the control or the LT heifers, whereas the LT heifers gained similarly to the controls. Plasma levels of TBOH rose to over 900 pg/ml in response to the implantation in both treated groups, but stabilized at a lower level in the ST group than in the LT group. In both groups plasma E2 beta rose to over pg/ml in response to TBA implantation; in fact, a regression analysis of E2 beta on TBOH in individual cows showed that the two hormones varied directly. Fat and liver accumulated substantial TBOH during implantation, but it cleared rapidly during the 15 d withdrawal period. Indications from this experiment are that TBA has promise for promoting rate of gain and efficiency of feed utilization in feedlot heifers. No undesirable effects on carcass quality were detected. The drug cleared blood and tissues rapidly after the implant was withdrawn. Further research is needed to determine the most effective implantation schedule. It would be desirable to determine whether a positive response to TBA will occur in animals being finished predominantly on forages.
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Some bodybuilders and athletes use trenbolone for its muscle-building and otherwise performance-enhancing effects. [ citation needed ] Such use is illegal in the United States and many other countries. The DEA classifies trenbolone as a Schedule III controlled substance under the Controlled Substances Act .  Trenbolone is classified as a Schedule 4 drug in Canada  and a class C drug with no penalty for personal use or possession in the United Kingdom .  Use or possession of steroids without a prescription is a crime in Australia .  The infamous "duchess" cocktail administered to Russian athletes at the Sochi Winter Olympics consisted of oxandrolone , metenolone , and trenbolone.