Melatonin should be used with caution when combined with medications, including: antidepressants, antianxiety medications, diabetes medications, narcotics, blood pressure medication, and asthma medication. If you are using these or any other medications, please consult with your physician prior to using melatonin.
Do not use melatonin unless advised by a physician if you are pregnant, attempting to become pregnant or nursing, or have a chronic disease, including hypertension (high blood pressure) or diabetes, or if you have an endocrine, auto-immune, depressive, bleeding, or seizure disorder. Do not take melatonin while operating a motor vehicle or machinery.
Cells are exposed to various concentrations of MG-132 for 24, and 48 hours. Supernatant and monolayer cells are harvested by centrifugation and fixed in 70% ethanol in PBS for staining with acridine orange. Equal volumes of cells and acridine orange (5 mg/mL in PBS) are mixed on a microscope slide and examined by fluorescence microscopy. For annexin V analysis, cells are harvested by centrifugation and stained with annexin V and propidium iodide. For cell cycle analysis, cells are rehydrated in PBS at room temperature for 10 minutes, followed by staining with propidium iodide (5 mg/mL). All samples are analyzed using a Coulter Epics XL flow cytometer.